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. Author manuscript; available in PMC: 2014 Jun 11.
Published in final edited form as: Cell Host Microbe. 2013 Dec 11;14(6):10.1016/j.chom.2013.11.004. doi: 10.1016/j.chom.2013.11.004

Figure 6. NK Cells Accumulate in the Bladder following UPEC CFT073 Infection.

Figure 6

(A) Percentages of PI-positive murine NK cells incubated at 37°C for 3 hr with wild-type hemolytic (CFT073, ATCC 25922, UTI89, UTI536) and nonhemolytic (XL1, SR71) UPEC bacteria, with the irrelevant mutant B5 or with the hemolysinA-deficient mutants (C93, D57, and E49). *p < 0.005 for CFT073, B5, ATCC 25922, UTI89, and UTI536 versus all other bacteria.

(B) Number of GFP-positive NK cells in the bladder on day 1 and on day 2 following infection with UPEC CFT073 or with the hemolysinA-deficient C93 mutant. Injection of PBS was used as control (five to eight mice were used in each group). *p < 0.005 for NK cells present in the bladders injected with C93 on day 2 versus day 1 and versus day 2-injected UPEC CFT073 bladders.

(C) Representative confocal microscopy images of uroepithelial cells obtained from murine bladders injected with PBS (top), with wild-type UPEC CFT073 (middle), or with the hemolysinA-deficient mutant C93 (bottom). NK cells are visualized by GFP. Left panels depict merge staining of DAPI-stained bladder cells with the GFP-labeled NK cells; right panels show the NK cells only. For (A) and (B), data are a representative of three independent experiments, and an average ±SD of triplicate is shown. Slides for confocal microscopy were prepared from four to eight bladders of mice in each group and were taken from two independent experiments on day 2 of UTI induction. 25922 stands for ATCC 25922. See also Figure S4.