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. Author manuscript; available in PMC: 2013 Dec 20.
Published in final edited form as: Cell Microbiol. 2010 Oct;12(10):10.1111/j.1462-5822.2010.01485.x. doi: 10.1111/j.1462-5822.2010.01485.x

Figure 4. Curli contribute to recognition of whole E. coli cells through TLR1/TLR2.

Figure 4

(A) Detection of CsgA expression in the indicated E. coli strains using Western blot with rabbit anti-CsgA serum. (B) Congo red binding activity of bacterial colonies formed by the indicated E. coli strains. (C, D, E and F) HeLa cells carrying a NFκB luciferase reporter were mock-transfected (vector) or transfected with the indicated pathogen recognition receptors. Cells were stimulated with the indicated E. coli strains (C, D and E) or with whole cell lysates of the indicated E. coli strains (F). Non-stimulated cells served as negative controls. Activity of the NFκB luciferase reporter was monitored by measuring RLU. Bars represent averages from at least three independent experiments ± standard error.