Figure 5. Co-induction of full fusion and transient fusion of Phytophthora zoospores vesicles during encystment.

(A) Localization of dorsal (green), large peripheral (red-yellow) and ventral (blue) vesicles and their contents within zoospores and a young cyst. (B) Behaviour of the three types of peripheral vesicles (i) before, (ii) during and (iii) after induction of regulated secretion. Dorsal (green) and ventral (blue) vesicles undergo full fusion, secreting their entire contents onto the spore surface. Large peripheral vesicles (red-yellow) undergo transient fusion during which PnCcp proteins (red) are selectively secreted and PnLpv (yellow) proteins are retained. After fission of the fusion pore, large peripheral vesicles move away from the plasma membrane.