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A putative iPSC colony with variegated mRFP1 expression was identified on day 7 (dashed white circle). On day 19, the well was stained with Alexa488-labeled antibody to Tra-1-60, a pluripotency marker, and Hoechst 33342 for visualizing nuclei, and then observed by phase contrast and fluorescence microscopy as described in the text.
