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(A) Untransduced and transduced MRC-5 fibroblasts (MRC-5 5V) were analyzed by flow cytometry and the percentage of transduced fibroblasts was determined after gating the untransduced cells to determine the ‘negative’ fraction. (B) Fluorescence and phase contrast images of untransduced and transduced MRC-5 cells. The percentage of transduced cells was determined using the cell-counter feature in NIH ImageJ software.
