Table 3. Description of iPSC primers used in RT-qPCR.
| Laboratory designation |
Description (nucleotide position) | Product size | Sequence (5′ – 3′) | Melt temp |
Parent GenBank Accession No |
|---|---|---|---|---|---|
| AT103 | Human β-Actin, S (897-916) | 84 | CTGTGGCATCCACGAAACTA | 59.7 | NM_001101 |
| AT104 | Human β-Actin, AS (961-980) | AGCACTGTGTTGGCGTACAG | 60.0 | ||
| AT105 | Human NANOG, S (347-366) | 88 | AGATGCCTCACACGGAGACT | 59.9 | NM_024865 |
| AT106 | Human NANOG, AS (415-434) | TTGGGACTGGTGGAAGAATC | 59.9 | ||
| AT75 | Human DNMT3B, S (1394-1413) | 203 | ATAAGTCGAAGGTGCGTCGT | 59.8 | NM_006892 |
| AT76 | Human DNMT3B AS (1577-1596) | GGCAACATCTGAAGCCATTT | 60.1 | ||
| AT111 | Human OCT4, S (750-771) | 146 | AAAGCGAACCAGTATCGAGAAC | 59.8 | NM_002701 |
| AT112 | Human OCT4, AS (879-898) | GCCGGTTACAGAACCACACT | 60.0 | ||
| AT175 | Human OCT4, S 3′UTR (1243-1262) | 55 | AGGAAGGAATTGGGAACACA | 59.4 | NM_002701 |
| AT176 | Human OCT4, AS 3′UTR (1279-1297) | AACCAGTTGCCCCAAACTC | 60.0 | ||
| AT65 | Human SOX2, S (1302-1320) | 151 | CCCAGCAGACTTCACATGT | 57.4 | NM_003106 |
| AT66 | Human SOX2, AS 3′UTR (1433-1452) | CCTCCCATTTCCCTCGTTTT | 57.8 | ||
| AT113 | Human SOX2, S (632-651) | 92 | GATGCACAACTCGGAGATCA | 59.8 | NM_003106 |
| AT114 | Human SOX2, AS (704-723) | GCTTAGCCTCGTCGATGAAC | 60.0 | ||
| AT115 | Human KLF4, S (2002-2021) | 110 | CACCTCGCCTTACACATGAA | 59.7 | NM_004235 |
| AT116 | Human KFL4, AS 3′UTR (2092-2011) | CATCGGGAAGACAGTGTGAA | 59.7 | ||
| AT117 | Human KLF4, S(1774-1793) | 67 | GCCACCCACACTTGTGATTA | 59.4 | NM_004235 |
| AT118 | Human KLF4, AS (1821-1840) | GTGCCTTGAGATGGGAACTC | 59.7 | ||
| AT177 | Human cMYC, S (1083-1102) | 94 | CTCCACCTCCAGCTTGTACC | 59.7 | NM_002467 |
| AT178 | Human cMYC, AS (1157-1176) | GCTGTCGTTGAGAGGGTAGG | 59.9 | ||
| AT179 | Human cMYC, S 5′UTR (119-138) | 74 | AGGGATCGCGCTGAGTATAA | 59.8 | NM_002467 |
| AT180 | Human cMYC, AS 5′UTR (173-192) | TGCCTCTCGCTGGAATTACT | 60.0 |
Notes.
- S
- sense
- AS
- antisense