Figure 10.

Orexin fails to produce Ca²⁺ transients in slices from DKO mice. (A) Somatic dF/F signals recorded from fura-2AM loaded LDT, DR, and LC cells in slices from DKO mice. Orexin-A application did not produce detectable changes in dF/F for any recorded cell. (B) Bolus application of glutamate (5 mM; delivered at arrow) produced strong Ca²⁺ transients in the same cells indicating that they remained viable. Horizontal bars above the traces indicate application of orexin-A (Orx-A; 300 nM). Calibration bars indicate 50% dF/F and 2 min in (A) and 50% dF/F and 1 min in (B).