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. 2013 Dec 20;7:246. doi: 10.3389/fnins.2013.00246

Figure 11.

Figure 11

Levels of whole brainstem OX2 mRNA are higher in OX−/−1 mice compared to wild-type mice. (A–C) Top Gel images of the amplicons resulting from probes for OX1 (A), OX2α (B), and OX2β (C). Samples for each lane were 1: blank, 2–5: scales for corresponding receptor, 6: C57 sample, 7: OX−/−1 sample, 8: OX−/−2 sample, 9: DKO sample. (A) Bottom RNA for OX1 (OX1 per total) was quantified from whole brainstems of C57BL6(C57; 28 samples from 14 mice), OX−/−1 (9 samples from 5 mice), OX−/−2 (28 samples from 14 mice), double orexin receptor knockouts (DKO; 8 samples from 4 mice) and 129SvEv (129s; 16 samples from 8 mice) mice by RT-PCR. OX1 mRNA was undetectable in tissue from OX1−/− and DKO mice. Despite significantly higher levels of OX1 mRNA in brainstems from 129s mice compared to C57 and OX−/−2 brainstems (*P < 0.0001), there was no significant difference between mRNA levels measured in brainstems from C57 and OX−/−2 mice. (B) Bottom mRNA levels for OX2α (OX2α per total) were measured from isolates obtained from the same mice used in (A). OX2α mRNA was undetectable in tissue from OX−/−2 (7 samples from 4 mice) and DKOs (20 samples from 10 mice). OX2α mRNA levels were higher in OX−/−1 samples (20 samples from 10 mice) than in either C57 (14 samples from 7 mice) or 129s (16 samples from 8 mice) samples (*P < 0.02). (C) Bottom Similarly, mRNA levels for OX2β (OX2β per total) were measured from mRNA isolates from the same mice in A. OX2β mRNA was undetectable in tissue from OX−/−2 (14 samples from 7 mice) and DKOs (20 samples from 10 mice). OX2β mRNA levels were higher in tissue from OX−/−1 mice (26 samples from 13 mice) than in tissue from either C57 (14 samples from 7 mice) or 129s mice (15 samples from 8 mice, *P < 0.02).