Figure 6.

Orexin augments voltage-dependent Ca²⁺ transients in nNOS+ LDT neurons, TpH+ DR neurons, and TH+ LC neurons in slices from OX^−/−₁ mice. Orexin-A augmented the voltage-dependent Ca²⁺ transient in identified neurons. Left column illustrates low-power fluorescent micrographs of the recorded slices immunostained with Alexa-488 (green) for nNOS in LDT (A), TpH in DR (B), and TH in the LC (C arrows). The second column illustrates a higher-power image of the recorded and fluorescently labeled neurons (Alexa-594) from each nucleus. The third column shows the same field with Alexa-488 visualized. The right column shows the images merged, revealing that each recorded neuron was immunopositive for nNOS, TpH, and TH, respectively.