Figure 9.

Inhibition of PKC by Bis I attenuated Ca²⁺-transients evoked by activation OX₁ or OX₂. (A) Ca²⁺ transients in LDT, DR, and LC neurons in slices from OX^−/−₂ mice were attenuated by prior application of Bis I. (B) Similarly, Ca²⁺ transients in LDT, DR, and LC neurons in slices from OX^−/−₁ mice were attenuated by prior application of Bis I. Horizontal bars above the traces indicate application of orexin-A (300 nM). Calibration bars indicate 20% dF/F and 2 min in each panel.