Table 1.
Vector phage | Phage virion proteins for fusion display | Copy number of display | Size of display shown |
---|---|---|---|
M13 | gpVIII | ≈2700 | Small peptide (6–8 amino acids) |
gpIII | ≈5 | Large protein with reduced viability | |
T4 | Soc | ≈960 | Large protein (up to 837 amino acids) |
Hoc | ≈160 | Protein (up to 183 amino acids) | |
T7 | 10A | ≈415 | Protein (40–50 amino acids) |
10B | ≈1 | Protein (up to 1200 amino acids) | |
Lambda (λ) | gpD | ≈420 | Large protein (1024 amino acids) |
Data were taken from Smith (1985), Markland et al. (1991), Mikawa et al. (1996), Ren et al. (1996, 1997), Ren and Black (1998), Iwasaki et al. (2000), Savinov and Austin (2001), Sche et al. (2001), Gupta et al. (2003), Pacheco et al. (2006), and Li et al. (2007).
The above phages are used for presentation of displayed antigens to mammalian cells; the λ system was also developed as a DNA delivery platform to antigen-presenting cells (APC).