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Reduction in RMRP-S1 and RMRP-S2 follows Dicer knockdown. (A) TaqMan assays measuring the time course of knockdown of RMRP-S1 and -S2 after Dicer knockdown. Black bars: RMRP-S1, gray bars: RMRP-S2. 48 h RMRP-S2 was not determined. (B) Time course of knockdown of Dicer mRNA after siRNA transfection determined by QRT-PCR assay. Fold difference = 2(−ddCt)+ _SEM (n = 6 for each sample). M = mock; D24, D48 and D72 = 24, 48 and 72 h post-transfection with Dicer siRNA.
