Figure 1.

Adult heterozygous Chd7 mice have fewer mature OB interneurons. (A) Schematic sagittal view of an adult mouse brain showing the location of coronal sections through the OB in (C, D, F, G, I and J) (dotted line, OB). (B) Schematic of a DAPI-stained coronal section through the OB denoting the location of panels (C, D, F, G, I and J) in the GL and GCL (orange box). Immunolabeling with anti-CALB (C and D), anti-CALR (F and G) and anti-TH (I and J) antibodies in wild-type and Chd7 heterozygous null (Chd7^Gt/+) mice shows 35 and 48% reductions in the number of CALB+ and TH+ interneurons in the GL, respectively, and 15% reduction in the number of CALR+ interneurons in the GCL of Chd7^Gt/+ mice compared with wild type (E, H and K). Scale bars in (C and D), (F and G), (I and J): 75 μm. Error bars in (E, H and K) indicate standard error of the mean (SEM) (n = 3 per genotype). *P < 0.05, ***P < 0.001 by unpaired Student's t-test. EPL: external plexiform layer