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. 2013 Sep 10;23(2):434–448. doi: 10.1093/hmg/ddt435

Figure 7.

Figure 7.

CHD7 binds to the promoters of RA receptor and pro-neuronal transcription factor genes and regulates their expression. (A) qRT-PCR analysis of total RNA from P1 control and Ubc Cond KO microdissected SVZ shows expression levels of Rarb, Rxrg, Neurod1 and Sox2. Threshold cycles are normalized to Gapdh. In Ubc Cond KO P1 SVZ, expression levels of Rarb, Rxrg and Neurod1 are decreased by 55–58% compared with controls. Sox2 expression is unchanged in Ubc Cond KO SVZ compared with controls. Black dashed line denotes control gene expression levels. (B–D) By ChIP-qPCR, CHD7 is enriched relative to IgG at conserved regions 1, 2, 4, 6 and 8 for Rarb (B), conserved regions 1, 3, 4 and 6 for Rxrg (C) and conserved regions 1–4 for Neurod1 (D). Non-conserved regions are not enriched for CHD7 relative to IgG. (E) Proposed model showing CHD7 function in neural progenitors, including regulation of Rarb, Rxrg and pro-neuronal gene transcription and promotion of differentiation into neuroblasts. Chd7 deficiency results in decreased Rarb, Rxrg and pro-neuronal Neurod1 gene transcription leading to fewer neuroblasts and a shift toward glial fates. Error bars in (A–D) indicate SEM. *P < 0.05.