Fig. 6. Figure 6.

Mutant CKIε is deficient in PERIOD phosphorylation (87). S-agarose beads containing either recombinant wild-type or tau mutant CKIε enzyme were incubated with in vitro– translated, ³⁵S-methionine-labeled substrate and washed extensively. Binding and kinase reactions are indicated for both substrates. Following exposure to detect both the ³⁵S and ³²P signals, dried gels were covered with two layers of exposed X-OMAT film to block the ³⁵S signal. PER signal is indicated by filled triangles.