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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Nat Struct Mol Biol. 2013 Sep 8;20(10):1164–1172. doi: 10.1038/nsmb.2659

Figure 4. Degradation activities for base variants containing single-subunit pore-loop mutations.

Figure 4

Degradation of a polyubiquitinated GFP fusion substrate was monitored by the loss of fluorescence upon addition of proteasomes reconstituted with base variants containing (a) YA mutations in the Ar-Φ loop of single Rpt subunits, and (b) DN mutations in the pore-2 loop of single Rpt subunits. Degradation activities under saturating conditions were measured relative to reconstituted proteasome containing wild-type (WT) recombinant base. Errors were estimated to be ±10% (s.d.) of the mean WT value based on repeat measurements (n = 3 technical replicates). The circular diagrams are alternative representations, with the line thickness corresponding to the observed degradation activities for a mutation in the respective subunit.