(A) Total spleen monocytic cells from ARM or C13 infected mice were compared to the frequency of IFN-γ+ GP33-specific CD8+ T cells.
(B) Flow cytometry sorted monocytic cells from day 14 post LCMV infection were cultured with OT-I splenocytes in the indicated ratios. Cultures were stimulated with no peptide, SIINFEKL or vaccinia (B8R) peptide. Proliferation was determined by [3 H] Thymidine incorporation.
(C-D) Sorted MDSC from C13 were cultured 1:2 with purified OT-1 T cells and stimulated in the presence of NOS or ARG1 inhibitors or blocking antibodies. Proliferation was measured by CFSE dilution after 3 days of culture.
Percent proliferation was calculated relative to stimulated OT-I splenocytes without added MDSC. Mean ± SEM are performed with 4 experiments performed in triplicate (B) or 3 independent experiments (C-D). *p<0.05, **p<0.01, ***p<0.001.