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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: J Mol Cell Cardiol. 2013 Oct 21;65:10.1016/j.yjmcc.2013.10.007. doi: 10.1016/j.yjmcc.2013.10.007

Figure 1. Proteoglycan expression is reduced in atrioventricular canal regions isolated from post natal Scx−/− mice.

Figure 1

(A) qPCR analysis to show fold changes in proteoglycan gene expression in atrioventricular canal regions isolated from post natal Scx−/− mice compared to wild type littermate controls. * p<0.05 using Student’s t-test, n=4. (B–E) Immunohistochemistry to detect chondroitin sulfate proteoglycan (CSPG) expression (green) in mitral valves (arrows, B, C) and atria (D, E) from post natal wild type (Scx+/+)(B, D) and Scx−/−(C, E) mice. Blue indicates DAPI-stained cell nuclei, red indicates wheat germ agglutinin staining (cell membranes). mv, tricuspid valve.