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. 2013 Dec 1;2(6):448–454. doi: 10.1089/biores.2013.0046

FIG. 2.

FIG. 2.

Process for bioprinting spheroids. The human SVF cell population is mixed in 1.5% alginate, and this cell suspension is placed in a delivery pen. Extrusion of fluid is controlled by increasing the pressure in the delivery pen to a specific value, causing a droplet to form. The delivery pen is then lowered toward the CaCl2 solution at a rate of 5 mm/sec. The suspended droplet subsequently overcomes the surface tension, and a spheroid forms within the CaCl2 solution.