FIG. 1.

Uptake and intracellular distribution of Ce6 in 4T1 cells. (A) Alterations of Ce6 fluorescence intensity in 4T1 cell at various Ce6 concentrations with different incubation time was measured by flow cytometry. Data are presented as mean±SD from three independent experiments. Ce6 concentration: ■, 5 μg/mL; □, 2 μg/mL; ▲, 1 μg/mL; △, 0.5 μg/mL; ●, 0 μg/mL. (B) After incubation with 1 μg/mL Ce6 for 4 hours, cells were stained with 3 nM MT-G (mitochondria probe) and 1 μg/mL Ho (nucleus dye). After co-loading, cells were visualized by confocal microscopy to evaluate intracellular distribution of Ce6 in 4T1 cells.