Table 1.
Scoring Matrix
| Parameter | Time point | Smart Matrix vs. CCG | Smart Matrix vs. Integra | Smart Matrix vs. Matriderm |
|---|---|---|---|---|
| Matrix contraction |
2 |
1 |
0 |
0 |
| |
7 |
1 |
0 |
0 |
| Cell viability+proliferation (alamarBlue assay) |
2 |
0 |
0 |
1 |
| |
7 |
0 |
0 |
0 |
| Cell viability+proliferation (Ki67 expression) |
2 |
0 |
0 |
0 |
| |
7 |
0 |
0 |
0 |
| Apoptosis (annexin V staining) |
2 |
1 |
0 |
1 |
| |
7 |
1 |
0 |
0 |
| Morphology (SEM) |
2 |
1 |
0 |
0 |
| |
7 |
1 |
0 |
0 |
| Morphology (H&E) |
2 |
1 |
1 |
1 |
| |
7 |
1 |
1 |
1 |
| α-SMA expression |
2 |
1 |
0 |
1 |
| |
7 |
0 |
0 |
0 |
| Cytokine and growth factor expression profile | ||||
| VEGF |
2 |
0 |
0 |
0 |
| |
7 |
0 |
0 |
0 |
| bFGF |
2 |
0 |
0 |
0 |
| |
7 |
0 |
0 |
0 |
| TGFβ |
2 |
0 |
0 |
0 |
| |
7 |
0 |
0 |
0 |
| TNFα |
2 |
0 |
1 |
0 |
| |
7 |
0 |
0 |
0 |
| IL-6 |
2 |
0 |
0 |
0 |
| |
7 |
0 |
0 |
0 |
| IGF1 |
2 |
0 |
0 |
0 |
| |
7 |
0 |
0 |
0 |
| Leptin |
2 |
0 |
0 |
0 |
| |
7 |
0 |
0 |
0 |
| EGF |
2 |
0 |
0 |
0 |
| |
7 |
0 |
0 |
0 |
| Total | 9 | 3 | 5 | |
CCG, contractile collagen gel; SEM, scanning electron microscopy; H&E, hematoxylin and eosin; α-SMA, alpha-smooth muscle actin; VEGF, vascular endothelial growth factor; bFGF, fibroblast growth factor b; TGFβ, transforming growth factor beta; TNFα, tumor necrosis factor alpha; IL, interleukin; IGF1, insulin-like growth factor 1; EGF, epidermal growth factor.