Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Effect of MTX and AZA on induction of IDO activity by ASCs. ASCs were stimulated with IFN-γ (3 ng/mL) in the absence or presence of MTX (100 μg/mL) or AZA (1 μM), and concentration of tryptophan and kynurenine was determined by HPLC at different time points. Mean and standard deviation of four experiments are shown. Similar results were observed with MTX (1000 μg/mL) and AZA (10 μM). IDO, indoleamine 2,3-dioxygenase; IFN, interferon.
