FIG. 4.

MTX and AZA did not affect the generation of Treg cells by ASCs. PBMCs were cultured alone or with ASCs and stimulated with IL-2 (100 U/mL) in the absence or presence of MTX (0.01 or 100 μg/mL) and AZA (1 or 10 μM). The percentage of Treg population (defined as CD4⁺CD25⁺Foxp3⁺ cells) was measured at 7 days. White bars: PBMCs without ASCs. Black bars: PBMCs with ASCs. Fold of induction for each condition relative to the corresponding control without ASCs is also represented. n=3. Treg cells, regulatory T cells; IL, interleukin.