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MTX and AZA did not alter the expression of immunogenic markers on ASCs. ASCs were stimulated or not with IFN-γ (3 ng/mL) in the absence or presence of 100 μg/mL of MTX (A) or 1 μM of AZA (B) and the expression of HLA-I, HLA-II, CD80, and CD86 was determined by flow cytometry after 72 h. Specific antibodies (gray peak) and isotype controls (white peak). One representative experiment is shown (n=6).
