FIG. 8.

Analysis of oxidative stress markers in the nongenetic mouse model. (A, B) IHC showed increased expressions of 8-OHdG (A) and 4-HNE (B) in mice fed the HFrD and injected with STZ (HFrD+STZ, n=10 mice per group). The numbers of 8-OHdG- and 4-HNE-positive hepatocytes (arrowheads) were significantly greater in the NAFLD livers from WT mice than in the livers from Tg mice. *p<0.05. C, central vein. Original magnification:×200. Scale bar=80 μm. (C) Serum TBARS levels expressed as nmol MDA/mg LDL protein were significantly lower in Tg mice than in WT mice after 8 weeks of HFrD feeding and STZ injection (HFrD+STZ, n=10 mice per group). *p<0.05. Values are means±SE. 4-HNE, 4-hydroxy-2-nonenal; 8-OHdG, 8-hydroxy-2′-deoxyguanosine; MDA, malondialdehyde; TBARS, thiobarbituric acid reactive substances. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars