FIG. 1.
(A) Representative in vivo image depicting the procedure for a direct diaphragm injection. The left hemidiaphragm is exposed, and a 40 μl intramuscular injection is given in the diaphragm using a 29G needle with a bent tip. (B) LacZ-stained diaphragm depicting the area of distribution after direct intramuscular diaphragm injections of 1×1011 vg rAAV-LacZ. Arrows depict approximate injection sites. rAAV, recombinant adeno-associated virus. Color images available online at www.liebertpub.com/hgtb