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(A and B) Representative direct GFP detection in diaphragm muscle. (A) Cross section of a noninjected, control diaphragm. (B) Cross section of a diaphragm injected with 1×1011 vg AAV9-CMV-GFP. (C and D) Representative hematoxylin and eosin staining of diaphragm muscle. (C) Noninjected, control diaphragm. (D) Injected (diaphragm intramuscular injection).
