FIG. 2.

Examination by Sanger sequencing of error-prone NHEJ at HPRT1 alleles of human myoblasts transduced with ZFN-encoding vectors. LV clone series: nucleotide sequences of independent XhoI-treated PCR products obtained by using primers Fwd.H1.Ex3 and Rev.H1.Ex3 (Fig. 1B) on DNA from myoblasts cotransduced with LV.ZFN-1^HPRT and LV.ZFN-2^HPRT. IDLV clone series (ID+): nucleotide sequences of individual XhoI-treated PCR products obtained by deploying the aforementioned primers on DNA from TSA-exposed human myoblasts cotransduced with IDLV.ZFN-1^HPRT and IDLV.ZFN-2^HPRT. The ZFN target site sequences are boxed, and the spacer nucleotides are in lower case.