Analysis of mouse B7-H3 binding to TLT-2 and T cells. B7-H3-Fc fusion protein to the surface of T cells (stimulated by PHA at 0, 6, 12, 24, and 48 h, respectively) (A) and TLT-2 transfected cells (B). Cells were stained with mouse B7-H3-Fc (open histograms) or negative control human IgG (grey histograms). Data shown are representative of three independent experiments.