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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: J Mol Cell Cardiol. 2013 Aug 27;64:10.1016/j.yjmcc.2013.08.003. doi: 10.1016/j.yjmcc.2013.08.003

Figure 3.

Figure 3

Contractility measurements in isolated adult mouse cardiomyocytes. (A) Basal sarcomere shortening of cardiomyocytes paced at 0.5Hz. (B) ISO-induced sarcomere shortening. Sarcomere shortening was measured 5 minutes after 1, 10, or 100 nmol/L ISO treatment. (C) Effects of PDE inhibitors on sarcomere shortening. Cardiomyocytes were pretreated with Mil (10 μmol/L) or rolipram (Rol, 10 μmol/L) for 10 minutes followed by ISO (10 nmol/L) stimulation. Sarcomere shortening was measured 5 minutes after isoproterenol stimulation. Values mean ± SEM represent averages of 6-10 cells from 3 mice per condition. *P<0.01 compared to WT.