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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: J Mol Cell Cardiol. 2013 Aug 27;64:10.1016/j.yjmcc.2013.08.003. doi: 10.1016/j.yjmcc.2013.08.003

Figure 4.

Figure 4

Cardioprotective effect of PDE3A1 overexpression against ischemia/reperfusion (I/R) injury. (A) Representative photographs of post-I/R mouse heart slices perfused with Evans Blue and stained with triphenyltetrazolium chloride (TTC). Mice were subjected to myocardial ischemia for 45 min and reperfusion for 24 hours through ligation of the left anterior descending artery. Hearts were then perfused with 2% Evans Blue to delineate the area at risk (AAR), transversely cut into 4-5 slices, and stained with TTC to access the myocardial infarct area (MI). AAR was the unstained area by Evans blue dye and MI was the unstained area by TTC. (B-C) Quantification of percentage of AAR in the total LV or MI in AAR, AAR and MI were assessed using NIH Image J software. (C) Quantification of TUNEL positive nuclei in AAR of the corresponding mice. (D) LV ejection fraction of pre and post I/R assessed by M-mode echocardiography. Values are mean ± SEM (n=5-8 each group). *P<0.05 vs. WT.