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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: J Mol Cell Cardiol. 2013 Aug 27;64:10.1016/j.yjmcc.2013.08.003. doi: 10.1016/j.yjmcc.2013.08.003

Figure 6.

Figure 6

Apoptotic analysis of isolated adult cardiomyocytes. Adult mouse cardiomyocytes were isolated from ventricles of WT or TG mice and subjected to normoxia (control) or hypoxia/reoxygenation (H/R) (hypoxia for 30 min and reoxygenation for 24 hours) or treated with 1 μM H2O2, in the presence of absence of 30 μM of milrinone. Apoptotic myocytes were detected by TUNEL staining. Myocytes with nuclear TUNEL staining were counted. (A-B) Quantitative data showing the percentage of TUNEL positive myocytes. Values are mean ± SD (n=5 for WT and n=4 for TG). For each sample, 200-400 myocytes were counted. * P<0.05, ns: no significant difference.