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. 2013 Dec 20;8(12):e82234. doi: 10.1371/journal.pone.0082234

Figure 4. Autoradiographic analysis of CrPV IGR IRES-mediated translation in coupled eukaryotic cell-free systems.

Figure 4

The influence of the CrPV IGR IRES on protein expression of the secreted protein Mel-eYFP (29 kDa; A) as well as the membrane proteins Mel-Hb-EGF-eYFP (51 kDa; B) and Mel-EGFR-eYFP (162 kDa; C) was investigated in coupled systems based on lysates from Sf21, CHO and K562 cells. Plasmids encoding the target protein were equipped with (+) or without (−) the CrPV IGR IRES (GCU) in the EasyXpress pIX3.0 vector backbone. 14C-leucine-labeled, de novo synthesized proteins were visualized by autoradiography after gel electrophoresis.