Skip to main content
. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Mol Cancer Res. 2013 Oct 23;11(12):10.1158/1541-7786.MCR-13-0294. doi: 10.1158/1541-7786.MCR-13-0294

Figure 4. Transplantation of BRAFV600E bone marrow in sublethally-irradiated recipients.

Figure 4

A. Kinetics of circulating leukocyte counts (top), hemoglobin values (middle) and platelet counts (bottom) of sub-lethally-irradiated Rag2−/−γc−/− mice transplanted with 2×106 bone marrow cells from BRAFV600E mice (V600E BMT; filled circles, n=14) or control mice (Ctrl BMT; open circles, n=9), or injected with PBS only (Sham BMT; open squares, n=3). Peripheral blood was monitored at 3, 5, 7, 9, 11 and 13 weeks after transplantation. B. Representative Mac1/Gr1 FACS plots of circulating leukocytes in the recipients injected with PBS (Sham BMT, top) or transplanted with control (Ctrl BMT, middle) or BRAFV600E (V600E BMT, bottom) bone marrow. C. Spleen weights in the recipients injected with PBS (Sham BMT), control bone marrow (Ctrl BMT) or BRAFV600E bone marrow (VE BMT) at 10-13 weeks after transplantation. D. Flow cytometry quantification of Mac1+Gr1high (granulocytes), Mac1+Gr1low/− (monocytes), TER119+ (erythroid) and CD19+ (B-lymphocytes) populations in bone marrow (top) and spleen (bottom) of control (Ctrl BMT) or BRAFV600E (V600E BMT) bone marrow recipients at 10-13 weeks after transplantation. P-values by student’s t-test are indicated. E. Representative CD71/TER119 FACS plots of Mac1-negative spleen cells in control (Ctrl BMT) or BRAFV600E (V600E BMT) bone marrow recipients. F. Myeloid colony formation of spleen cells from control (Ctrl BMT) or BRAFV600E (V600E BMT) bone marrow recipients in mechylcellulose media with or without multiple cytokines (as in Figs. 3A and D). Data in D and F represent mean +/− SD (n=6 for V600E, n=3 for Ctrl).