Table 1. Bone marrow progenitor colony formation and chimerism in transplant recipients.

Recipient mouse#	Colony #/105 BM cells		Chimerism (+3STE)(c)
	No cytokine	+3STE(b)	% Donor chimerism	Recipient CFU# /105	Donor CFU# /105
BRAFV600E BMT(a)
103	0	85	6.7	79.3	5.7
106	9	92.5	6.7	86.3	6.2
114	31	42.5	53.3	19.8	22.7
118	15	117.5	0	117.5	0
122	9	80	15.4	67.7	12.3
127	33.5	72.5	93.3	4.8	67.7
Average	16.3	81.7	29.2	62.6	19.1
Control BMT (a)
116	0	165	46.7	88	77
124	0	190	26.7	139.3	50.7
128	0	225	53.3	105	120
Average	0	193.3	42.2	110.8	82.6

^(a)2×10⁶ bone marrow nucleated cells from BRAF^V600E or control mice 3 weeks after poly I:C injection were injected into each sublethally (5 Gy) irradiated Rag2^−/−γc^−/− recipient.

^(b)10ng/ml IL-3, 50ng/ml SCF, 100ng/ml TPO and 4U/ml EPO were added in “+3STE” cultures.

^(c)Donor chimerism was determined by Braf genotyping of randomly plucked individual colonies developed in “+3STE” cultures, and CFU# of donor or recipient origin was calculated by multiplying total colony numbers by the %chimerism.