Figure 6. Anoxic preconditioning (APC) prevents HKII dissociation from mitochondria during anoxia in NRVM.

A & B) Snapshots of fluoresence in NRVM during anoxia, illustrating rapid translocation of HKII-YFP from mitochondria to cytosol (A), which is prevented if NVRM are exposed to 3 short anoxic preconditioning exposures (1 min each) before the prolonged anoxia episode (B). C & D) Time course of changes in spatial variance (C) versus the ratiometric ROI method (D). For the ratiometric ROI methods, a ROI with a high concentration of mitochondria (1) was compared to a ROI placed into a region with few mitochondria (2) as illustrated in the series of images in A & B. Note that the measurement time points on differents cells varied, explaining the different number of measurements for each time point.