Figure 7. A) Fluorescence snapshots of AVRM expressing HKI-YFP (upper) or HKII-YFP under control conditions and after different periods of anoxia. Note that the mitochondrial network is very dense and uniformly dispersed, making difficult to define appropriate ROI if we wanted to apply the ratiometric ROI method. B) Corresponding spatial variance images. C) Average changes in spatial variance during anoxia, confirming that HK2, but not HK1, translocates to the cytoplasm during anoxia. Note that the measurement time points on differents cells varied, explaining the different number of measurements for each time point.
