Table 1. Variable nucleotide positions in gatB (A) and amino acid positions in GATB (B) of wCer2.

variable nucleotide positions in gatB of w Cer2	3	11	16	31	32	36	42	61	72	88	89	93	102	112	121	149	186	195	206
consensus	T	A	G	C	T	C	T	A	A	T	A	T	T	A	A	C	T	A	A
w Cer2 R. cerasi	.	.	.	.	.	.	C	G	G	.	.	.	.	.	.	T	.	G	.
w Cer2 RC21	.	.	.	.	.	.	.	.	.	.	.	.	.	G	.	.	.	.	.
w Cer2 RC20	.	.	.	.	.	.	C	.	.	.	.	C	C	.	T	.	C	.	.
w Cer2 RC33	.	.	.	.	A	.	.	.	.	.	G	.	.	.	.	.	.	.	.
w Cer2 RC45	C	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
w Cer2 RC50	.	G	.	.	.	.	.	.	.	C	.	.	.	.	.	.	.	.	.
w Cer2 Wol Med88.6	.	.	A	T	.	A	.	.	.	.	.	.	.	.	.	.	.	.	G
SNP-Frequency	singleton	2× in RC	singleton	singleton	singleton	singleton	3× in RC, 1× in R. cerasi	singleton	singleton	singleton	singleton	2× in RC	singleton	singleton	singleton	singleton	2× in RC	singleton	singleton
variable nucleotide positions in gatB of w Cer2	221	226	238	250	253	257	288	305	321	323	324	343	350	354	355	367	371	390	398
consensus	T	C	T	A	C	T	T	T	A	A	A	A	A	A	A	T	T	A	A
w Cer2 R. cerasi	C	.	.	.	.	.	.	.	G	.	G	.	.	.	.	.	.	.	.
w Cer2 RC21	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
w Cer2 RC20	.	T	.	.	.	.	.	C	.	G	.	G	.	.	.	.	.	.	.
w Cer2 RC33	.	.	.	G	.	.	.	.	.	.	.	.	.	.	.	C	.	.	.
w Cer2 RC45	.	.	C	.	.	C	C	.	.	.	.	.	.	G	.	.	.	C	.
w Cer2 RC50	.	.	.	.	T	.	.	.	.	.	.	.	.	.	G	.	C	.	.
w Cer2 Wol Med88.6	.	.	.	G	T	.	.	.	.	.	.	.	G	.	.	.	.	.	G
SNP-Frequency	singleton	singleton	singleton	1× RC, 1× in WolMed88.6	1× RC, 1× in WolMed88.6	singleton	singleton	singleton	singleton	singleton	singleton	singleton	singleton	singleton	singleton	singleton	singleton	singleton	singleton
variable amino acid positions in GATB of w Cer2	1	4	6	11	11	12	14	21	24	30	30	31	34	38	41	50	62	65	69
consensus	A	E	V	L	L	L	S	M	R	Y	Y	I	C	M	G	F	R	I	N
aa changes in all RC lines and Wol Med88.6	.	G	I	F	R	.	.	V	G	H	C	.	.	V	.	S	.	M	S
variable amino acid positions in GATB of w Cer2	74	76	80	84	85	86	96	102	107	108	108	115	117	118	119	121	124	130	133
consensus	I	Q	D	R	E	I	S	F	G	K	K	L	D	A	S	Y	F	L	E
aa changes in all RC lines and Wol Med88.6	T	*	.	G	*	T	.	S	.	R	.	E	E	.	G	H	S	F	G

A) Position 1 in the presented 404 bp fragment corresponds to position 981 of the full gatB locus of wRi infecting Drosophila simulans Riverside (GenBank accession number CP001391). Aa position 1 in (B) corresponds to aa position 148 of the full GATB protein of wRi (protein ID:ACN94961.1). Frequency of SNP indicates which SNPs are singletons or occur recurrently in what host system. Nonsense mutations leading to a stop codon are indicated by asterisks. Abbreviations: aa amino acid.(