Figure 3. Characterization of LAAO activities from Pseudoalteromonas sp. R3 (R3-LAAO) with L-Leu and L-Met as substrates, respectively (A) and from Crotalus adamanteus venom (caLAAO) with L-Leu as substrate (B) based on the Fe^IIIXO agar assay.

On the basis of the diameters of the formed purplish red Fe^IIIXO halos, the concentrations of H₂O₂ produced by LAAO activities were calculated with the equations in Figure 2. The corresponding standard H₂O₂ solutions as indicated above the corresponding holes were used to confirm the accuracy of Fe^IIIXO agar assay. The diameters of the purplish red halos were marked below the holes. Without R3-LAAO, both L-Leu and L-Met (negative controls) did not give purplish red Fe^IIIXO halos (right holes).