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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Cell Signal. 2013 Sep 12;26(1):10.1016/j.cellsig.2013.08.043. doi: 10.1016/j.cellsig.2013.08.043

Fig. 1.

Fig. 1

HER3 associates with MTK1 in response to HRG stimulation. A schematic representation of the proline-rich region (PPP), actin-interacting region (AIR), alanine-rich region (AAA), and catalytic domain of MTK1 is shown. The MTK1 antibody recognizes an amino terminal region (amino acids 18–139) of MTK1 (Panel A). T-47D cells were stimulated with 10 nM HRG for 12 min or 0.3 M sorbitol for 30 min as indicated (Panel B). MTK1 was immunoprecipitated and proteins were resolved by SDS-PAGE and immunoblotted using monoclonal antibody 4G10 (pTyr). T-47D cells were stimulated with HRG and MTK1 was immunoprecipitated as described above (Panels C & D). Immunoblots were performed using antibodies directed against EGFR and HER2 (Panel C) or HER3 and HER4 (Panel D). Membranes were stripped and immunoblotted with MTK1 antibody (B, C and D, bottom panels).