Figure 1. RNA Aptamer Isolation via Dual-cycles (RAPID).

(A) Diagram of the RAPID process. The starting library or the enriched pool from the previous selection step can either go through the (inner) Non-Amplification Cycle and be used immediately in the next selection or go through the regular (outer) Amplification Cycle. (B) An example of processing times for SELEX and RAPID to complete two full selection cycles. Each selection is indicated with black blocks and arrowheads (▾) on top. (C) The total time required to complete six cycles of SELEX under optimal enrichment conditions, and six cycles of RAPID performed by alternating between Non-Amplification and Amplification Cycles; each colored block represents the total processing time between amplification steps. Asterisks (*) indicate the enriched and amplified pools that were analyzed via high-throughput sequencing.