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. 2013 Dec 20;8(12):e82667. doi: 10.1371/journal.pone.0082667

Figure 2. Binding of RNA after each selection cycle.

Figure 2

(A) Percent RNA recovery for SELEX cycles for Empty (orange circles), UBLCP1 (red squares), and CHK2 (blue triangles) microcolumns. In this mode, there is a clear distinction between the protein-bound and the Empty microcolumns. (B) Percent RNA recovery for RAPID cycles for the same targets. In this mode, there are significant increases in the percent aptamer recoveries following selections with non-amplified pools at Cycles 2, 4, and 6, followed by a concentration induced drop with the amplified pools at Cycles 3 and 5. (C) Test of enriched pool binding to CHK2 protein preparation. F-EMSA shows the progression of bulk binding affinity increase for both SELEX and RAPID enriched pools with the RAPID Cycle 6 pool showing higher bulk binding than the SELEX Cycle 6 pool.