Figure 4. The rad54-AA mutant protein is deficient in most of its biochemical activities.

A. Rad54-AA has lower ATPase activity compared to wild type Rad54. Rad54-AA and Rad54 wt (75 nM, each), respectively, were mixed with dsDNA and α-[³²P]-labeled ATP. At indicated times, samples were withdrawn and analyzed by thin-layer chromatography. Error bars represent standard error produced by 3 experiments. B. Rad54-AA does not branch migrate mobile Holliday junctions. DNA substrate was incubated with increasing concentrations (2.5, 5, 10, 20 nM) of Rad54 wt (lanes 2–5) or Rad54-AA (lanes 6–9), respectively, in the presence of ATP. Lane 1 shows the no protein control reaction.