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. 2013 Dec 20;8(12):e82652. doi: 10.1371/journal.pone.0082652

Figure 3. Silencing of the GFP is maintained in grapevines grown at low temperatures.

Figure 3

(A) Silencing of the GFP is maintained in somatic tissues of GFFG+GFP transgenic grapevines grown at 4°C. (B) Quantitative real-time PCR of the GFP mRNA in GFP expressing or GFP-silenced grapevines grown at 26°C, 15°C or 4°C. mRNA levels were normalized to that of the reference ubiquitin gene for grapevine. (C) Semi-quantitative analysis of the fluorescence in GFFG+GFP transgenic grapevines or GFFG/GFP Arabidopsis grown at 26°C, 15°C, 10°C or 4°C. Fluorescence intensity is represented as grey level intensity/pixel. Means and standard deviations are calculated from at least 3 experimental repetitions of 5 different plants.