Figure 1. Experimental protocol (modified Rice-Vannucci model) for inducing and confirming the HI insult in P2 pups.
(A) Ligation of the right common carotid artery in isoflurane-anesthetized pups (i) was followed by maintenance into the hypoxic chamber (5% O2/95% N2) at 37°C for a variable period of time (120–200 min) (ii). (B) Confirmation of the HI episode at P2 by angiography and arterial spin labeling. (i) TOF angiogram in axial section at subcranial level demonstrating missing blood flow of the right common carotid artery. (ii) Maximal intensity projection of whole TOF data set showing diminished or missing blood flow of cerebral arteries. (iii) ASL imaging in coronal section maps perfusion deficits in the ligated (right) hemisphere.