Figure 3. Nicotine deteriorates the osteogenic differentiation capacity of hPDLSCs.
A: hPDLSCs treated by 10−4–10−6 mol/L nicotine were cultured in osteogenic medium and osteogenic differentiation was determined by Alizarin red S staining after 21 days. Representative entire plate views of alizarin red staining in 6-well plates for PDLSCs from each group. B: The quantity result for Alizarin Red S staining; C: The expression of osteogenic related genes Runx2, BSP, ALP and OCN were determined by qRT-PCR after 14 days of culturing with osteogenic supplement. D: The expression of osteogenic related protein Runx2, BSP, ALP and OCN were determined by western blot after 14 days of culturing with osteogenic supplement. Data represent the means ± SD. *p<0.05 (n = 3).