Skip to main content
. 2013 Dec 20;8(12):e83102. doi: 10.1371/journal.pone.0083102

Figure 5. Nicotine deteriorates the osteogenic differentiation of hPDLSCs through α7 nAChR regulating Wnt/β-catenin pathway.

Figure 5

A: The expression of wnt signaling pathway related proteins were determined by western blot, wnt signaling pathway was activated by nicotine in the downstream of α7 nAChR; B, C: hPDLSCs treated with/without nicotine and dkk1 were cultured in osteogenic medium and osteogenic differentiation was determined by Alizarin red S staining after 21 days. D: The expression of osteogenic related genes Runx2, BSP, ALP and OCN were determined by qRT-PCR after 14 days of culturing with osteogenic supplement. E: The expression of osteogenic related proteins Runx2, BSP, ALP and OCN were determined by western blot after 14 days of culturing with osteogenic supplement. Data represent the means ± SD. *p<0.05 (n = 3).