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. 2013 Dec 20;8(12):e83453. doi: 10.1371/journal.pone.0083453

Figure 6. Mediastinal lymph node cell stimulation during chlamydial Infection.

Figure 6

WBCs harvested from mediastinal lymph nodes were stimulated with heat killed chlamydial antigen and resulting cytokine secretions revealed that cells from infected adult mice secreted elevated levels of IFN-g and IL-2 when compared to infected neonates and controls (A, B). Cells harvested from infected neonatal mice secreted predominantly Th2 (IL-5, 4, 10, and 13) cytokines in response to chlamydial antigen compared to infected adults and control groups (C-F). WBCs from infected neonatal mice 14 days and 28 days post infection produced significantly elevated levels of IL-5 (P = 0.0353, 0.0456 respectively, C) and IL-4 (P = 0.0444, D) when compared to WBCs from infected adults. Cells were also stimulated with PBS and J774A.1 proteins as controls (data not shown).