Table 1. Velvet assembly result is sensitive to read coverage.

Percentage of total reads used in assembly	Number of reads	Coverage per nt	Number of MIRNA containing contigs (length>1000 bp)	MIRNAs recovered
4.0%	993,695	80	0	None
2.0%	497,844	40	2	MIR166f and MIR165b
1.0%*	248,489	20	7	All but MIR166c/d
0.5%*	124,550	10	8**	All but MIR165b
0.25%*	62,474	5	6	All but MIR165a, MIR165b

^*At these levels of read coverage, three independent read sampling and assembly experiments were performed. All results were consistent.

^** MIR166c and MIR166d were assembled into separate contigs, despite their ∼2 kb distance. See Table S4 for details.