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. 2013 Dec 20;8(12):e83620. doi: 10.1371/journal.pone.0083620

Figure 1. HCMV infection of endothelial cells reduces SIRT1 and FOX3A expression in endothelial cells.

Figure 1

A. QRT-PCR analysis of SIRT1 and FOXO3A mRNA in HUVECs at the indicated times after infection and the relative amount of mRNA was normalized to β-actin. SIRT1 or FOXO3A mRNA level from mock infected ECs serves as reference. B. Western blot analysis of SIRT1 and FOXO3A protein level in HUVECs was performed using equal protein loading of mock-infected and HCMV-infected. The histogram shows relative density of SIRT1 and FOX3A compared to mock infection. Values are expressed as mean±SD from three independent experiments, *p <0.05 vs. controls.